通过调节因素激活II类基因转录被一种新型活动增强
M Meisterernst1, A L Roy, H M Lieu
1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Cell
|September 6, 1991
概括
确定了一种新的激活剂依赖转录因子,USA. 它包括两个组件,通过与TFIID相互作用来调节转录,增强激活器功能.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 基因法规 基因法规
背景情况:
- 转录激活剂通过与基底转录机械相互作用来调节基因表达.
- 在开始转录过程中,TFIID复合体起着至关重要的作用.
研究的目的:
- 描述一种新型活动 (美国) 刺激激活剂依赖转录.
- 阐明美国影响转录启动的机制.
主要方法:
- 使用纯化的组件重建的转录系统.
- 凝移动性转移试验用于研究蛋白质-DNA和蛋白质-蛋白质相互作用.
- 新活动的净化 (美国).
主要成果:
- 美国的激活剂依赖转录在与天然TFIID复合的系统中被刺激了10到50倍.
- 美国在被分解成两个组成部分时,对核心促进体活性产生相反影响的潜在激活器功能.
- 凝移动转移实验揭示了USA和TFIID的抑制成分之间的物理相互作用.
结论:
- 美国含有与预启动复合体相关联的辅因子.
- 这些辅因子降低基底促进体活性,并增强激活体依赖转录.
- 美国代表了转录启动的新型监管机制.
相关概念视频
Meiosis II
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Meiosis II
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Meiosis II
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Chromatin Modification in iPS Cells
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...


