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Updated: Jun 28, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
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Published on: September 27, 2011

可光切换的纳米粒子使高分辨率的细胞成像成为可能:PULSAR显微镜.

Dehong Hu1, Zhiyuan Tian, Wuwei Wu

  • 1Pacific Northwest National Laboratory, Richland, Washington 99352, USA.

Journal of the American Chemical Society
|October 23, 2008
PubMed
概括

研究人员开发了光触发的单分子逻辑切换实现的重建 (PULSAR) 显微镜,用于超出衍射极限的成像. 这种新的技术使用可光切换的螺旋纳米粒子来实现细胞结构的纳米尺度分辨率.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.