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需要Lid2来协调H3K4和H3K9对异色染色素和 euchromatin的甲基化
Fei Li1, Maite Huarte, Mikel Zaratiegui
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. feili@nature.berkeley.edu
Cell
|October 30, 2008
概括
JmjC域蛋白 Lid2 作为一个组分体 H3 氨-4 (H3K4) 脱甲基酶,对异色素蛋白的形成和基因沉默至关重要. Lid2将H3K4脱甲基化与H3K9甲基化联系在一起,协调色素调节.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
- 分子生物学分子生物学
背景情况:
- 基因组甲基化模式,特别是H3K9和H3K4甲基化,对于分别定义异色染色素和欧色染色素至关重要.
- 这些修改决定了真核生物中的染色质结构和基因表达调节.
研究的目的:
- 为了确定S. pombe中JmjC域蛋白Lid2的功能.
- 阐明Lid2在调节基因组甲基化中的作用及其对染色质结构的影响.
主要方法:
- 生物化学测试以确定Lid2作为脱甲基酶的酶活性.
- 基因分析Lid2功能在异性染色素的形成和沉默.
- 研究Lid2,Clr4和Dos1/Clr8-Rik1复合体之间的蛋白质-蛋白质相互作用.
主要成果:
- Lid2被确定为一个三甲基H3K4脱甲基酶,负责H3K4在色素中低甲基化.
- 通过Dos1/Clr8-Rik1复合体,Lid2在物理和功能上与H3K9甲基转移酶Clr4相互作用.
- 破坏Lid2的JmjC域导致异染色素缺陷和siRNA枯竭,而Lid2的过度表达增强了沉默.
- Lid2也需要用于euchromatin中H3K4和H3K9甲基化的交叉调节.
结论:
- Lid2在协调H3K4和H3K9甲基化中扮演着双重角色,用于适当的异色素蛋白形成和基因沉默.
- 这些发现揭示了H3K4脱甲基化和H3K9甲基化之间的机制联系,突出了协调的表观遗传调节.
- 在 euchromatin 中Lid2 的活性可能是通过与其他基因组修饰酶的动态相互作用来调节的.
相关概念视频
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
01:01Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
01:01Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...