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一个快速,坚固和可调节的合成基因振荡器
Jesse Stricker1, Scott Cookson, Matthew R Bennett
1Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.
Nature
|October 31, 2008
概括
在大肠杆菌中设计了一个快速,坚固,可调节的基因振荡器,周期短至13分钟. 这种合成生物学进步表明了创建稳定的基因调节网络的关键设计原则.
科学领域:
- 合成生物学 合成生物学
- 基因工程是一种基因工程.
- 系统生物学 系统生物学
背景情况:
- 合成生物学旨在利用计算模型设计基因调节网络.
- 像切换开关和振荡器这样的基本基因电路已经开发并应用于各种环境中.
- 坚固且可调节的基因振荡器对于精确的细胞控制至关重要.
研究的目的:
- 在大肠杆菌中设计出一种快速,坚固和持久的基因振荡器.
- 调查设计原则,以创建稳定和可调节的基因调节网络.
- 通过对工程电路的实验观测来验证计算模型.
主要方法:
- 设计了一个基因振荡器,使用连接正负反循环的计算模型.
- 使用微流体平台进行单细胞显微镜和精确的环境控制.
- 通过光在单个细胞中的多个循环中监测细胞振荡.
主要成果:
- 实现了一个可调节的基因振荡器,振荡周期最快13分钟.
- 在几乎所有观察到的细胞中表现出显著的稳定性和振荡的持久性.
- 表明振荡周期可以通过诱导器水平,温度和介质组成来调整.
结论:
- 负反循环的时间延迟对于强大的振荡器设计至关重要.
- 积极的反增强了振荡的稳定性和可调性.
- 没有正反的简化振荡器设计得到了计算预测和实验证实.
相关概念视频
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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
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02:08Gene Conversion
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01:09Recombinant DNA
Overview
02:05Gene Evolution - Fast or Slow?
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...