一种类似于真核细胞的Ser/Thr激酶向细菌发出信号,让它们在对糖碎片的反应中退出休眠状态
Ishita M Shah1, Maria-Halima Laaberki, David L Popham
1Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Cell
|November 6, 2008
概括
休眠的 Bacillus subtilis 子可以通过从生长中的细菌中释放的木质来触发发芽. 对于这一过程来说,一种特定的激酶是必不可少的,为抗菌药物开发提供了一个新的目标.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 子的发芽 子的发芽
背景情况:
- 细菌进入休眠状态以生存恶劣的条件,需要环境线索恢复生长.
- 细菌的生长通常是通过释放细胞壁碎片 (muropeptides) 的信号.
研究的目的:
- 为了研究muropeptides在休眠的Bacillus subtilis子的发芽中的作用.
- 为了确定分子机制和宿主因子参与muropeptide诱导的子发芽.
主要方法:
- 用muropeptides治疗休眠的 Bacillus subtilis 子.
- 基因分析以确定重要的宿主因素.
- 使用小分子调节酶活性的生物化学测试.
主要成果:
- 墨是休眠的 Bacillus subtilis 子的强有力的发芽剂.
- 一种类似于真核细胞的Ser/Thr膜激酶具有细胞外糖结合域,对于感知muropeptides至关重要.
- 特定的小分子可以诱导或抑制依赖于muropeptide的子芽.
结论:
- 细菌子发芽可以通过通过muropeptides检测其他生长细菌的存在来触发.
- 鉴定到的激酶代表了开发抗微生物药物的新目标,这些抗微生物药物特别抑制子发芽,与传统抗生素不同.
- 稳氨酸通过阻断这种激酶来抑制子发芽,这表明针对休眠的细菌形式的新策略.
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