脂质体封装的抗原在溶酶体中被加工,回收利用,并呈现给T细胞
C V Harding1, D S Collins, J W Slot
1Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Cell
|January 25, 1991
概括
抗原是在溶解体中生成的,而不是早期的内分体,用于向T细胞呈现. 这种溶解体处理通过MHC-II分子增强了抗原呈现.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 抗原处理对于T细胞识别和免疫反应至关重要.
- 第二类主要基因相容性复合体 (MHC-II) 分子呈现出来自T辅助细胞的细胞外或内体抗原的.
- 对MHC-II呈现的抗原代谢的精确细胞内位置进行了辩论.
研究的目的:
- 为了确定MHC-II呈现发生抗原处理的特定细胞内区.
- 调查溶解体在产生MHC-II加载的作用.
主要方法:
- 蛋溶酶被封装在对酸敏感或抗酸脂质体中,以控制其释放位置.
- 脂质体的含量是针对早期内分体 (对酸敏感) 或溶解体 (抗酸) 的.
- 评估了抗原的处理和呈现,包括作为 lysosomal 处理的指标的 chloroquine 敏感性.
主要成果:
- 通过耐酸脂质体传递给溶酶体的抗原被有效地处理,并且比传递给早期内分酶体的抗原更有效地呈现.
- 对溶酶体向抗原的处理对洛昆敏感,证实了溶酶体的参与.
- 敏感脂肪体在早期内体中释放抗原,导致处理和呈现效率较低.
结论:
- 密度高的溶解体是产生免疫性的首要部位,该与MHC-II分子结合.
- 可以从溶解体回收到内分体,用于MHC-II加载和随后的细胞表面呈现.
- 溶酶体处理对于T细胞的最佳抗原呈现至关重要.
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