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14:40
Expression Analysis of Mammalian Linker-histone Subtypes
Published on: March 19, 2012
基底RNA聚合酶II转录的基底RNA聚合酶II转录的基因素H1介导的抑制
G E Croston1, L A Kerrigan, L M Lira
1Department of Biology, University of California, San Diego, La Jolla 92093.
Science (New York, N.Y.)
|February 8, 1991
概括
基因组H1作为基因转录的抑制剂. 序列特定的因素可以释放这种抑制 (抗抑制),有些也可以激活转录,揭示基因调节的独特机制.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因规则 基因规则
背景情况:
- 基因表达受到与DNA结合的转录因子的严格控制.
- 基因素H1是已知的转录抑制剂,影响色素结构.
- 了解抑制剂和激活剂之间的相互作用对于破译基因控制至关重要.
研究的目的:
- 通过促进剂/增强剂结合因子来研究转录控制的生物化学机制.
- 阐明转录因子如何调节RNA聚合酶II活性.
- 为了区分真正的转录激活和由序列特定因素介导的反压缩.
主要方法:
- 净化和识别一个一般的转录抑制剂作为基因素H1.
- 使用纯化的素H1与裸体DNA结合,作为抑制色素的模型.
- 评估Sp1,GAL4-VP16和GAGA因子对H1介导的抑制和基底转录的影响.
主要成果:
- 已证实,素H1是转录的一般抑制剂.
- Sp1,GAL4-VP16和GAGA因子表现出抗压缩活性,抵消H1.
- 在没有H1的情况下,Sp1和GAL4-VP16表现出真正的激活,这与反压缩不同.
结论:
- 基因组H1在保持基因组处于压抑的基本状态方面发挥着作用.
- 序列特定的转录因子利用基底抑制 (抗抑制) 的真实激活和释放来诱导基因表达.
- 通过GAL4-VP16介导的抗抑制可以在多个转录轮中持续存在,这表明稳定的调节相互作用.
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