位点定向的 рибо甲基化在多基化基质中识别了2'-OH组,这对于AAUAAA的识别和多A的添加至关重要
V J Bardwell1, M Wickens, S Bienroth
1Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706.
Cell
|April 5, 1991
概括
调查信使RNA (mRNA) 聚氨基化发现,AAUAAA序列内的特定糖接触对特异性因子的识别至关重要. 糖和的识别对于这种RNA-蛋白相互作用至关重要.
科学领域:
- 分子生物学分子生物学
- 处理RNA处理RNA处理
- 生物化学 生物化学
背景情况:
- 多基化是一种关键的转录后修改的真核细胞信使RNAs (mRNAs).
- 在3'未翻译区域的AAUAAA六合体序列是多基化的一个关键识别点.
- 在这种认识中,特定的RNA-蛋白相互作用的作用,特别是涉及糖分的作用,尚未完全理解.
研究的目的:
- 调查糖接触在mRNA多基化过程中对特定序列的识别的重要性.
- 确定在AAUAAA序列内特定位置的核糖甲基化对多基化效率和因子结合的贡献.
主要方法:
- 化学合成的RNA基质具有2'-O-甲基 (2'-O-CH3) 修饰在选定的核糖位.
- 采用这些改性RNA基质进行体外多化试验.
- 评估与修改后的RNA序列结合的特异性因子.
主要成果:
- 有效的多基化发生在RNA基质在所有位置甲基化,除了AAUAAA序列和3'-终端腺.
- 在AAUAAA序列内单个核糖酶的甲基化显著抑制了多 (A) 添加和特异性因子结合.
- 抑制的程度取决于核酸甲基化中的核酸位置,这表明糖接触的不同重要性.
结论:
- 特异性因子通过涉及基和糖分的RNA-蛋白接触来识别AAUAAA序列.
- 在AAUAAA内的不同核酸位的特定核糖接触对于高效的多基化是必不可少的.
- 识别机制涉及基特异性和糖特异性相互作用的组合,突出了RNA与蛋白质结合的复杂性.
相关概念视频
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
02:23RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
01:19RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...