母性遗传的piRNAs在转位子沉默中的表观遗传作用
Julius Brennecke1, Colin D Malone, Alexei A Aravin
1Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory (CSHL), 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Science (New York, N.Y.)
|November 29, 2008
概括
小RNA,特别是Piwi相互作用RNA (piRNA),对于表观遗传至关重要. 母亲遗传的piRNAs维持了Drosophila的生育能力,而它们的缺失导致了杂交异位.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 小RNA通过指导植物和哺乳动物的DNA甲基化来调解表观遗传.
- 小RNA可以作为表观遗传信息的直接载体.
- 在Drosophila中,当特定的转子被父继承时,混合失生导致不育.
研究的目的:
- 研究小RNAs在Drosophila的表观遗传中介作用.
- 为了确定混合异构的基础机制.
- 为了确定负责维持生育能力的因素.
主要方法:
- 对Drosophila后代中的Piwi相互作用RNA (piRNA) 含量进行分析,该后代来自涉及不同转子子菌株的交叉.
- 基于父母起源的piRNA配置文件的比较.
- 追踪piRNA从受精到成年期的持续性.
主要成果:
- 德洛索菲拉的女儿表现出不同的piRNA含量,针对特定的转子子,根据父母的起源而有所不同.
- 这些piRNA差异从受精时就被观察到,并在整个发育过程中持续存在.
- 母亲遗传的piRNAs对于有效的转子子沉默至关重要.
结论:
- 孕产妇沉积的piRNAs对于防止杂交失调产生至关重要.
- 母亲piRNA遗传的缺陷是Drosophila杂交异变的根本原因.
- 小RNAs作为表观遗传信息传输的直接载体.
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