在E mu-myc转基因小鼠中通过前病毒标记识别合作性瘤基因的识别
M van Lohuizen1, S Verbeek, B Scheijen
1Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
Cell
|May 31, 1991
概括
莫洛尼小鼠白血病病毒 (Mo-MLV) 感染加速了E-mu-myc小鼠的B细胞淋巴发育. 在Provirus标记中,发现了四种合作性瘤基因:pim-1,bmi-1,pal-1和bla-1.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- E mu-myc转基因小鼠是B细胞淋巴发育的模型.
- 莫洛尼小鼠白血病病毒 (Mo-MLV) 感染加快了这些小鼠的瘤发育.
- 识别合作基因对于理解B细胞转化至关重要.
研究的目的:
- 通过使用前病毒标记,识别与B细胞转化中的E mu-myc转基因合作的基因.
- 描述新的前病毒插入部位及其编码的蛋白质.
主要方法:
- 在感染Mo-MLV.的E mu-myc转基因小鼠中进行Provirus标记.
- 在B细胞前淋巴瘤中对前病毒插入部位的分析.
- 对bmi-1基因及其蛋白质产物的生物信息分析.
主要成果:
- 在35%,35%,28%和14%的瘤中分别发现了四个常见的前病毒插入位点 (pim-1,bmi-1,pal-1,bla-1).
- bmi-1,pal-1和bla-1代表了新的常见的前病毒插入部位.
- bmi-1基因编码了一种具有转录调节因子的保守核蛋白,包括一个指.
结论:
- 预病毒标记是有效的识别合作性瘤基因在淋巴发育.
- 鉴定到的位置,特别是bmi-1,pal-1和bla-1,在B细胞转化中具有重要意义.
- 需要进一步的研究来将基因分配给pal-1和bla-1并阐明它们的作用.
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