核基导向的粉样纳米管组件组件
Peng Liu1, Rong Ni, Anil K Mehta
1Center for Fundamental and Applied Molecular Evolution, Department of Chemistry, Emory University, Atlanta, Georgia 30322, USA.
Journal of the American Chemical Society
|December 5, 2008
概括
研究人员通过将细胞因子核基结合到粉样蛋白中,创造了新的纳米管. 这些自组装的ccAQLVFFA结构表现出一种富含β片的架构,通过互补的基体相互作用形成明确的纳米管.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 纳米技术纳米技术
背景情况:
- 氨基酸是众所周知的自我组装成β-sheet结构.
- 核基结合到中提供了新的功能可能性.
- 控制自组装是设计先进纳米材料的关键.
研究的目的:
- 为了合成和表征一种新型核基-联体 (ccAQLVFFA).
- 调查ccAQLVFFA的自组装行为进入更高阶结构.
- 探索细胞因子互补在指导纳米管形成中的作用.
主要方法:
- 体合成包括细胞因子核基.
- 用于结构分析的小角度X射线散射 (SAXS).
- 福利埃变换红外光谱 (FT-IR) 和X射线衍射 (XRD) 用于组装确认.
- 固态核磁共振 (NMR) 和线性二元化用于β-sheet注册表的确定.
主要成果:
- 在粉样片HHQALVFFA中成功结合了细胞素,产生了ccAQLVFFA.
- 在pH3-4下形成明确的纳米管 (外径24.8nm,壁厚3.3nm).
- 证实了具有交叉β架构的β片丰富组件.
- 氨基基基垂直于纳米管轴,通过互补相互作用增强β-sheet堆叠.
结论:
- 细胞因子功能化的粉样可以自组装成有序的纳米管.
- 核基在侧链中的互补相互作用推动了纳米管架构的形成.
- 这项工作扩大了信息编码的概念,超越了核酸双重体,进入了自我组装的共价网络.
相关概念视频
Assembly of Complex Microtubule Structures
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Amyloid Fibrils
Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Assembly of Cytoskeletal Filaments
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
Biosynthesis of Nucleic Acids
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...


