相关实验视频
Updated: Jun 27, 2026

07:14
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Published on: May 17, 2021
造血干细胞在平衡和修复过程中可逆地从休眠转变为自我更新
Anne Wilson1, Elisa Laurenti, Gabriela Oser
1Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland.
Cell
|December 9, 2008
概括
休眠的造血干细胞 (HSC) 被确定为强大的干细胞的储存库. 这些休眠的HSCs在压力期间可逆地进入细胞循环以产生血细胞,然后恢复休眠状态.
科学领域:
- 血液学 血液学 血液学
- 干细胞生物学 干细胞生物学
- 细胞循环规则 细胞循环规则
背景情况:
- 造血干细胞 (HSC) 对于终身的血细胞生产至关重要.
- 在平衡和压力期间,HSC分裂和自我更新的动态尚未完全理解.
- 之前的假设表明HSC的经常循环,这意味着细胞周期的进入和退出是正常的.
研究的目的:
- 在小鼠中识别和表征休眠的造血干细胞 (HSC).
- 为了研究在恒温和应激条件下HSCs的细胞周期行为.
- 确定休眠的HSC的自我更新能力和作用.
主要方法:
- 使用流式细胞计量来隔离特定的细胞群.
- 标签保留试验,包括BrdU和基因组H2B-GFP,用于追踪细胞分裂.
- 使用计算建模来估计细胞分裂频率.
主要成果:
- 在lin (((Sca1+cKit+CD150+CD48)) (((CD34)) 分数内确定了一个独特的休眠小鼠HSC (d-HSC) 种群.
- 计算机建模表明,d-HSCs大约每145天分裂一次.
- d-HSCs拥有大多数多系长期自我更新活动,并在骨髓损伤或G-CSF刺激期间被激活,在压力后恢复休眠状态.
结论:
- 造血干细胞 (HSC) 存在于休眠状态,形成一个强大的干细胞储备库.
- 在应对造血应激的过程中,HSCs可逆地在休眠状态和自我更新之间切换,而不是随机进入细胞周期.
- 这种可逆休眠机制确保了HSC池的长期维护.
相关概念视频
Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
Hematopoiesis
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Stem Cell Niche
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

