-异极体和同极体在相反的方向上曲DNA:对转录因子合作性的影响
1Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Cell
|July 26, 1991
概括
当Fos和Jun蛋白与AP-1部位结合时,它们会改变DNA螺旋结构. -异体和同体诱导不同的DNA曲线,形成独特的复合体.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- AP-1转录因子结合部位对于调节基因表达至关重要.
- 和蛋白形成异构体和同构体,它们与AP-1位点结合.
- 了解这些相互作用的结构后果是解读基因调节的关键.
研究的目的:
- 为了研究由Fos-Jun异构体和Jun同构体在DNA螺旋中诱导的构造变化.
- 为了确定由这些蛋白质复合体介导的DNA曲的方向和大小.
- 阐明 Fos 和 Jun 蛋白质的不同域如何促进 DNA 曲.
主要方法:
- 循环顺序分析,以评估由蛋白质结合诱导的DNA曲.
- 阶段分析以确定DNA曲的方向 (大或小槽).
- 使用全长蛋白质和截断的 (二分化和DNA结合域) 来绘制功能区域.
主要成果:
- 无论是Fos-Jun异构体还是Jun同构体,都会在AP-1位点诱导显著的曲.
- -异体体向主要沟方向曲DNA.
- 结合的同位体将DNA向小槽曲,表明相反的方向.
- 特定的蛋白质域会影响DNA曲角的大小.
结论:
- 尽管序列相似,但Fos-Jun异构体和Jun同构体在拓上形成不同的DNA-蛋白质复合体.
- DNA曲的方向是复杂的形成和功能的关键决定因素.
- 通过Fos-Jun和Jun同位体的差异性DNA曲有助于基因调节的特异性.
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