使用菌体显示库制作抗体片段.

科学领域:

分子生物学分子生物学
免疫技术是一种免疫技术.
生物技术是生物技术.

背景情况:

传统的混合体技术和动物免疫是产生抗体的常见方法.
这些方法可能耗时,昂贵,并引发道德问题.
开发替代的体外抗体生成策略至关重要.

研究的目的:

建立一种在细菌中产生抗体的方法,绕过动物免疫.
为了模仿抗体片段丰富的免疫选择过程.
探索菌体显示对抗体工程和亲和力成熟的潜力.

主要方法:

使用fd菌体显示器来呈现与较小的外衣蛋白融合的抗体碎片.
从用2-phenyloxazol-5-one (phOx) 免疫的小鼠中重新排列重 (VH) 和卡帕 (V卡帕) 轻链的随机组合图书馆.
采用触子亲和色谱来丰富表现出抗原结合抗体片段的菌体.

主要成果:

成功地在fd 菌体上显示了各种抗体碎片库.
在通过一个亲和系列后,识别了具有一系列phOx结合活动的菌体,包括高亲和度结合剂 (Kd = 10(-8) M).
通过连续传递证明了强结合剂对弱结合剂的丰富.
观察到V基因在结合剂中类似于anti-phOx杂交瘤,但是在新的,乱交的组合中.
通过将乱交的VH或V kappa基因与多样化的合作伙伴结合起来,分层图书馆的构建产生了更高亲和度的抗体配对.

结论:

菌体显示器为产生抗体提供了混合瘤技术的有效替代方案.
这种方法允许从具有改变V域配对的V基因库创建抗体.
这种方法有助于选择具有更好的结合亲和力的抗体.
通过菌体显示的细菌抗体生产为抗体工程提供了一个多功能平台.

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使用菌体显示库制作抗体片段.

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相关实验视频

使用菌体显示库制作抗体片段.

科学领域:

相关概念视频

相关文章

Direct integration of micro-LEDs and a SPAD detector on a silicon CMOS chip for data communications and time-of-flight ranging.

Gallium nitride micro-light-emitting diode structured light sources for multi-modal optical wireless communications systems.

Development of a sprayable hydrogel formulation for the skin application of therapeutic antibodies.

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背景情况:

研究的目的:

主要方法:

主要成果:

结论: