血管造血干细胞利基形成所需的内分体骨化是需要的
Charles K F Chan1, Ching-Cheng Chen, Cynthia A Luppen
1Department of Pathology, Developmental Biology and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, California, USA. chazchan@stanford.edu
Nature
|December 17, 2008
概括
成人造血干细胞 (HSC) 利基形成需要内分泌骨化. 特定的胎儿原生细胞驱动血管招募和骨髓腔生成,这对于HSC维护至关重要.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 血液形成 血液形成 血液形成
背景情况:
- 干细胞的维护依赖于特定的微环境,称为.
- 血造干细胞 (HSC) 利基形成的过程尚不清楚.
研究的目的:
- 开发一种用于成年HSC位形成的体内测试.
- 识别原生细胞种群对于高血压细胞利基发展至关重要.
主要方法:
- 采用了体内测试方法,将胎儿骨头前代细胞移植到成年老鼠囊下.
- 根据表面标记 (CD45,Tie2,alphaV,CD105,Thy1.1) 进行排序的原始细胞.
- 研究了内分离体骨化因子 (osterix,VEGF) 在利基形成中的作用.
主要成果:
- 鉴定了CD105(+) Thy1(-) 的原始细胞,这些细胞形成了带有骨髓腔的宫外骨,支持长期的HSC.
- 证明CD105(+) 基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因
- 表明抑制内分泌骨化因素会抑制利基基的产生.
- 证实,来自非内分泌体骨化区域的祖先不会形成髓腔.
结论:
- 带有软骨中间体的过程,即内分体骨化,对于成年HSC位形成至关重要.
- 特定的原生细胞种群 (CD105(+) Thy1(-)) 是通过内分泌骨化HSC利基发展的关键驱动因素.
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