德洛索菲拉和基因在受精时调节进入S阶段的基因
F L Shamanski1, T L Orr-Weaver
1Department of Biology, Massachusetts Institute of Technology, Cambridge.
Cell
|September 20, 1991
概括
德洛索菲拉母体基因和的突变导致DNA复制在未受精的卵子中过早开始. 这些基因对于调节DNA复制时间和早期发育中的核分裂至关重要.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 细胞循环规则 细胞循环规则
背景情况:
- 母亲基因控制Drosophila的早期胚胎发育.
- 适当的DNA复制和核分裂时间对胚胎发育至关重要.
研究的目的:
- 研究Drosophila母性基因 (plu) 和 (png) 的功能.
- 确定plu和png在调节DNA复制和核分裂中的作用.
- 分析png,plu和gnu之间的遗传相互作用.
主要方法:
- 对 plu 和 png 突变的多个等位基因的分析.
- 来自突变母亲的未受精和受精卵的表型分析.
- 基因相互作用研究.
主要成果:
- 和png中的突变导致未受精卵中的DNA复制启动.
- 在受精卵中,pl/png突变导致DNA复制从核分裂中脱离,形成多倍体核.
- 遗传相互作用显示,png,plu和gnu调节了相同的发育过程.
结论:
- 和png的野生类型功能需要抑制DNA复制,直到受精.
- png也可能在早期裂解分裂期间在S阶段和线粒分裂的合中发挥作用.
- 这三个基因 (png, plu, gnu) 在相同的调节途径中起作用.
相关概念视频
01:38Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.