在体外通过来自HIV-1 Rev的质对mRNA拼接的特殊调节
J Kjems1, A D Frankel, P A Sharp
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Cell
|October 4, 1991
概括
艾滋病毒-1的Rev蛋白通过与Rev响应元素 (RRE) 结合来抑制病毒mRNA拼接. 一种模仿Rev Rev的合成.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 处理RNA处理RNA处理
背景情况:
- 人类免疫缺陷病毒1型 (HIV-1) 的Rev蛋白对于调节病毒基因表达至关重要.
- Rev 促进从核到细胞质的未结合和部分结合的病毒mRNA的输出.
- 这种调节是由Rev与一种称为Rev响应元素 (RRE) 的特定RNA结构的相互作用介导的.
研究的目的:
- 研究Rev蛋白在体外调节mRNA前拼接的机制.
- 描述RevRNA结合域在拼接抑制中的作用.
- 为了确定Rev-mediated splicing规则的结构要求.
主要方法:
- 在体外剪接试验中,使用含有Rev响应元素 (RRE) 的前mRNA进行了分析.
- 一种合成的表征,模仿Rev.的RNA结合域.
- 对野生类型Rev和具有不同RRE结合特性的合成的拼接抑制的分析.
主要成果:
- 该Rev蛋白特别抑制含有RRE的mRNA前拼接的3至4倍.
- 一种含有Rev RNA结合域的合成17-氨基酸抑制了多达30倍的拼接.
- 的多个结合点可以替代RRE,表明多价值相互作用的重要性.
- 该的抑制作用发生在拼接复合体的组装过程中,这表明它干扰了拼接酶体的形成.
结论:
- Rev的RNA结合域足以对拼接进行特定的抑制.
- Rev介导的拼接抑制涉及与RRE上多个结合点的相互作用.
- Rev的基本区域的功能可能是防止功能拼接体的形成,从而调节病毒mRNA处理.
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