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Updated: Jun 22, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Published on: January 27, 2012

一个比度pH报告器用于成像活细胞中的蛋白质染色体结合体

Junyan Han1, Aurore Loudet, Rola Barhoumi

  • 1Department of Chemistry, Texas A&M University, Box 30012, College Station, Texas 77841, USA.

Journal of the American Chemical Society
|January 17, 2009
PubMed
概括

一个新的pH敏感的光探针可视化酸性细胞区. 这种能量传输探测器在中性pH下呈现绿色光,在酸性内体中呈现红色光,使细胞环境的清晰成像成为可能.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...