双向促进剂在酵母中产生普遍的转录
Zhenyu Xu1, Wu Wei, Julien Gagneur
1European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Nature
|January 27, 2009
概括
研究人员分析了酵母中的普遍转录,确定了稳定的未注释转录 (SUT) 和神秘的不稳定转录 (CUT). 这些非编码RNAs来自特定的基因组区域,揭示了促进者.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 细胞基因组表现出无处不在的转录,产生许多新的非编码RNA,如稳定的未注释转录 (SUT) 和神秘的不稳定转录 (CUT).
- 这些转录通常与注释的基因组特征重叠,引发了关于它们的起源和潜在功能的问题.
- 缺乏对完整的转录组架构 (包括SUT和CUT) 的全面理解.
研究的目的:
- 在Saccharomyces cerevisiae中全面分析SUT和CUT的全基因组分布和排列.
- 调查这些转录和基因组特征 (如促进体和核细胞自由区域 (NFRs)) 之间的关系.
- 阐明普遍转录对基因组组织和基因调节的影响.
主要方法:
- 在各种条件下对酵母中的SUT和CUT进行全基因组分析.
- 包括来自外体机械突变和不同酵母菌株背景的数据.
- 与注释基因和NFRs相关的转录启动站点的映射.
主要成果:
- SUTs和CUTs表现出不同的分布模式,经常从与其他转录的促进器或3'端相关的NFRs开始.
- 大约一半的编码转录也从与其他促进体相关的NFR开始.
- 这表明促进子双向性是基因调节的固有特征.
结论:
- 这项研究揭示了一种新型的转录组架构,其特点是普遍的转录和重叠的转录.
- 这些发现表明,促进者本质上是双向的,有助于广泛的重叠转录.
- 这种安排可能会通过转录干扰和基因组修饰等机制促进邻近基因的协调调节.
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