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广泛的双向促进体是酵母酵母中神秘转录的主要来源
Helen Neil1, Christophe Malabat, Yves d'Aubenton-Carafa
1Institut Pasteur, Unité de Génétique des Interactions Macromoléculaires, CNRS, URA2171, 75015 Paris, France.
Nature
|January 27, 2009
概括
研究人员在酵母中绘制了神秘的不稳定转录 (CUT),揭示了来自发起者的广泛,非随机转录. 这表明真核细胞促进体本质上是双向的,一些CUT可能会调节基因表达.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 单核细胞转录 单核细胞转录
背景情况:
- 隐藏的转录,包括神秘的不稳定转录 (CUTs),在真核生物中很常见,但不太了解.
- 切割细胞是RNA聚合酶II的转录,是Nrd1-外因子-TRAMP复合体的快速降解的目标.
- 之前的研究缺乏高分辨率,全基因组的CUT分布和流行率数据.
研究的目的:
- 创建Saccharomyces cerevisiae中CUTs的第一个高分辨率的基因组图.
- 调查CUTs的起源,流行和潜在的功能意义.
- 探索真核细胞促进体活动的性质,包括双向性.
主要方法:
- 对在CUTs中高度丰富的RNA部分的分析.
- 基因表达3'长序列分析 (SAGE) 进行深度测序.
- 生成一个详细的,核酸分辨率的CUTs的基因组图.
主要成果:
- CUTs源于明确定义的转录单元,而不是随机噪音.
- CUTs的转录主要发生在无核细胞体区域,通常重叠基因促进器区域.
- 一个CUTs子集在mRNA的上游启动,这表明在基因表达中具有潜在的调节作用.
- 大多数已识别的CUTs来自促进体的分离转录,表明其固有的双向性.
结论:
- 细胞促进体本质上是双向的,产生了感觉和分歧的转录.
- 不同的转录通常会产生短暂的CUT,这解释了为什么以前低估了促销器双向性.
- 该研究确定了许多新的CUT,其中一些在基因调节中具有潜在作用.
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