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Updated: Jun 26, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

通过间隙结合反应和表面杂交检测的高特异性和敏感的电化学基因造型

Yong Huang1, Yan-Li Zhang, Xiangmin Xu

  • 1State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Journal of the American Chemical Society
|January 31, 2009
PubMed
概括

这项研究引入了一种使用酶反应和表面杂交进行敏感和特定DNA分析的新电化学基因型定型方法. 该技术准确检测单核酸变异,为基因组分析提供了通用平台.

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相关概念视频

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...