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人类奥克卢丁是一种型肝炎病毒的进入因子,它是感染小鼠细胞所必需的
Alexander Ploss1, Matthew J Evans, Valeriya A Gaysinskaya
1Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA.
Nature
|February 3, 2009
概括
人类奥克卢丁 (OCLN) 被确定为肝炎C病毒 (HCV) 进入细胞的关键因素. 这一发现推动了小动物模型的开发,用于研究HCV感染和潜在的治疗方法.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 型肝炎病毒 (HCV) 导致严重的全球性肝病.
- 缺乏合适的小型动物模型阻碍了抗病毒疗法和疫苗开发.
- 冠状病毒热带性仅限于人类和黑猩猩,未知入口决定因素.
研究的目的:
- 为了确定肝炎C病毒进入细胞中必不可少的宿主因素.
- 了解HCV热流的特定物种限制.
- 促进用于HCV研究的小动物模型的开发.
主要方法:
- 代补充性DNA图书馆选. 这是一个很好的方法.
- HCVpp (HCV伪颗粒) 和HCVcc (细胞培养产生的HCV) 感染测定.
- 在小鼠和人类细胞系中,基因过度表达和沉默 (siRNA).
主要成果:
- 人类奥克卢丁 (OCLN) 被确定为HCV进入的关键因素.
- OCLN表达使小鼠细胞易受HCVpp感染.
- OCLN对于HCV进入人类和小鼠细胞是必要的,其第二个细胞外循环中具有特定物种的决定因素.
- 有效的HCV进入需要人类的OCLN和CD81,以及小鼠或人类的SR-BI和CLDN1.
结论:
- 奥克卢丁是一种新型和必不可少的宿主因子,用于C型肝炎病毒进入细胞.
- 紧结复合体在病毒进入过程中起着至关重要的作用.
- 对OCLN的鉴定是创建HCV的功能性小动物模型的重要一步.
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