染色体的特征揭示了哺乳动物中超过一千个高度保存的大型非编码RNA
Mitchell Guttman1, Ido Amit, Manuel Garber
1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature
|February 3, 2009
概括
研究人员在小鼠细胞中鉴定出超过1600个保存的大型干预非编码RNA (lincRNAs). 这些功能性lincRNAs在关键的细胞过程中扮演着不同的角色,挑战了以前关于转录噪声的概念.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 哺乳动物细胞产生大量的大型基因间转录,但它们的功能意义仍在争论中.
- 大多数以前识别的转录缺乏进化保护,这表明它们是转录噪声.
研究的目的:
- 开发一种用于识别功能性大型非编码RNA的新方法.
- 描述这些新发现的RNAs的进化保护和生物学作用.
主要方法:
- 利用染色体状态图来识别蛋白质编码基因之间的离散转录单元.
- 应用功能基因组学和基于细胞的测试用于功能预测和验证.
- 分析了基因组位置,外基因序列和促进区域的进化保护.
主要成果:
- 在四种小鼠细胞类型中识别了大约1600个大型多异构RNA (lincRNAs).
- 对于这些lincRNAs来说,已经证明了高进化保存率 (>95%),与之前的发现形成鲜明对比.
- 对lincRNAs分配了假定功能,揭示了胚胎干细胞多能性和细胞增殖中的作用.
- 通过 p53,NFkappaB,Sox2,Oct4 和 Nanog 等关键转录因子显示了 lincRNAs 的转录调节.
结论:
- 定义了一个独特的高度保存和功能性的lincRNAs集合.
- 确定了lincRNAs在各种生物过程中的重要性,超越了转录噪声的概念.
- 为进一步研究lincRNA功能和调控机制提供了基础.
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