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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding01:22

Protein Folding

Overview

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相关实验视频

Updated: Jun 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

类似原生RNA的三级结构使用了编码序列的裂变剂,并通过离散的分子动力学进行了细化.

Costin M Gherghe1, Christopher W Leonard, Feng Ding

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Chemical Society
|February 6, 2009
PubMed
概括

这项研究引入了一种新的计算方法,与RNA结构探测实验相结合,以准确地建模复杂的RNA三级结构. 这种方法在没有先前假设的情况下有效地改进RNA结构,有助于研究灵活且功能重要的RNA.

更多相关视频

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

相关实验视频

Last Updated: Jun 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

科学领域:

  • 生物化学 生物化学
  • 计算生物学 计算生物学
  • 结构生物学 结构生物学

背景情况:

  • 分析高阶RNA结构,特别是柔性域和折叠中间体,是一项挑战.
  • 现有的方法与RNAs进行斗争,需要对功能进行结构灵活性.
  • 准确的RNA三级结构建模对于理解RNA功能至关重要.

研究的目的:

  • 开发一种简洁而准确的方法来建模RNA的三级结构.
  • 为了克服灵活RNA的传统结构方法的局限性.
  • 为了使结构确定没有关于RNA折叠的先前假设.

主要方法:

  • 使用简单的RNA结构探测实验,包括二次结构的SHAPE化学.
  • 采用序列导向的切割剂来生成残余间距离信息.
  • 用快速,粗的离散分子动力学算法解释实验数据.
  • 用伪原子 (酸盐,核糖,核基) 代表每个RNA核酸.

主要成果:

  • 成功提炼了酵母tRNA中的基配对位置 (Asp) 到4 Å根-平方平均偏差 (rmsd).
  • 在没有预先存在的结构信息的情况下实现了高分辨率的结构改进.
  • 在没有用户干预的情况下,展示了一种优化了分辨率和速度的计算算法.

结论:

  • 这种混合的实验和计算方法为RNA结构建模提供了一个强大的新工具.
  • 该方法有可能为多样化,功能性重要的RNA产生与原生类型的模型.
  • 它解决了研究常规结构生物学技术难以处理的RNA的新方法的需要.