特定的突触在新皮质的姐妹刺激神经元中优先发展
Yong-Chun Yu1, Ronald S Bultje, Xiaoqun Wang
1Developmental Biology Program, Memorial Sloan Kettering Cancer Centre, 1275 York Avenue, USA.
Nature
|February 11, 2009
概括
放射性克隆中的新生刺激神经元在发育中的新皮层中形成特定的连接. 这种克隆组织有助于在成熟的大脑中形成功能性神经元柱.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞神经科学 细胞神经科学
背景情况:
- 哺乳动物新皮质神经元组织成具有特定突触连接的功能柱.
- 新皮质突触连接是稀疏和特定的,使相邻神经元能够独立处理信息.
- 在单个神经元层面的柱状架构底层的微电路构造尚未完全理解.
研究的目的:
- 调查发育中的新皮质中激发神经元的辐射克隆是否构成特定微电路的基础.
- 确定辐射克隆中的姐妹神经元是否建立了优先的突触连接.
主要方法:
- 在小鼠新皮质中使用增强绿色光蛋白 (EGFP) 表达逆转录病毒的激发性神经元的标记性ontogenetic辐射克隆.
- 进行多个电极全细胞记录以分析在产后发育期间姐妹神经元和非兄弟之间突触形成.
主要成果:
- 辐射对齐的姐妹刺激神经元显示出形成单向化学突触的倾向.
- 姐妹神经元之间的突触连接表现出在成熟的新皮质中观察到的相同的内膜方向偏好.
- 这些连接在姐妹神经元之间比在姐妹神经元和相邻的非兄弟姐妹之间更为普遍.
结论:
- 特定的微电路在发育中的新皮层中偏好地在激发神经元的辐射克隆中发展.
- 本体遗传辐射克隆作为一个基质的功能柱状微型架构的出现.
- 这一发现揭示了新皮层柱状组织的细胞基础.
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