综合的多态性调查阐明了Saccharomyces cerevisiae的种群结构
Joseph Schacherer1, Joshua A Shapiro, Douglas M Ruderfer
1Lewis-Sigler Institute for Integrative Genomics, Department of Ecology and Evolutionary Biology and Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey 08544, USA.
Nature
|February 13, 2009
概括
这项研究调查了63种Saccharomyces cerevisiae菌株的基因组变异,确定了数百万个单核酸多态和数千个删除. 这为酵母基因组广泛的关联研究提供了基础,并揭示了对酵母化和起源的洞察力.
科学领域:
- 基因组学就是基因组学.
- 人口遗传学 人口遗传学
- 微生物学 微生物学
背景情况:
- 识别遗传多态性对于理解表型变异和物种进化至关重要.
- 之前的大规模多态性调查已经在人类,小鼠和Arabidopsis thaliana中进行过.
研究的目的:
- 进行核酸水平的基因组变异的调查,在一个多样化的Saccharomyces cerevisiae菌株的集合.
- 建立基因组广泛的酵母协会研究资源.
- 研究Saccharomyces cerevisiae的种群结构和进化史.
主要方法:
- 来自63种Saccharomyces cerevisiae菌株的基因组DNA被杂交成全基因组片微阵列.
- 在整个基因组中检测到单核酸多态 (SNP) 和大删除事件 (>200个基对).
- 分析了整个基因组中的核酸多态,删除变异和链接不平衡的模式.
主要成果:
- 确定了189万个单核酸多态和101,343个不同的分离点.
- 检测到3985个超过200个基对的删除事件.
- 分析揭示了多态和删除的全基因组模式,并测量了链接不平衡.
结论:
- 生成的多态资源是未来在酵母中进行全基因组关联研究的基础.
- 这项研究提供了Saccharomyces cerevisiae多个化事件的证据.
- 获得了对致病性酵母菌株起源的洞察.
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