在Bacillus subtilis中,没有墙壁或划分机器的生命.
M Leaver1, P Domínguez-Cuevas, J M Coxhead
1Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle Upon Tyne NE2 4HH, UK.
Nature
|February 13, 2009
概括
科学家们发现了一种新的方式,使没有细胞壁 (L型) 的细菌能够繁殖. 一个单一的基因突变使得Bacillus subtilis能够形成L型,这些L型通过挤出而繁殖,而不是典型的细胞分裂.
科学领域:
- 微生物学 微生物学
- 细菌细胞生物学 细菌细胞生物学
- 抗生素耐药性研究研究
背景情况:
- 细菌细胞壁对保护至关重要,是抗生素的点.
- L型是缺壁的细菌,很难产生,而且不太了解.
- 它们在抗生素耐药性和病原发生中的作用仍然在很大程度上是未知的.
研究的目的:
- 开发一种可控制的系统,用于在Bacillus subtilis中产生L型.
- 为了确定细菌易于L型转变的遗传因素.
- 为了阐明L形传播的机制.
主要方法:
- 在Bacillus subtilis中开发可控制的L型生成系统.
- 基因组测序以确定与L型稳定性相关的突变.
- 显微镜和基因分析来研究L型的传播机制.
主要成果:
- 确定了一种单点突变,该突变使Bacillus subtilis倾向于L型生长.
- L型的传播并不依赖于法典的FtsZ-依赖的分裂.
- 观察到一种新型的挤出分辨机制用于L型增殖.
结论:
- 在Bacillus subtilis中建立了稳定的L型生成的遗传基础.
- L型细菌使用一种独特的繁殖方法,与墙体细菌不同.
- 这一发现为原始细胞增殖和潜在的抗生素耐药性机制提供了洞察力.
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