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相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...

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相关实验视频

Updated: Jun 25, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

mRNA定位:在空间维度中的基因表达.

Kelsey C Martin1, Anne Ephrussi

  • 1Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA 90095-1737, USA. kcmartin@mednet.ucla.edu

Cell
|February 26, 2009
PubMed
概括
此摘要是机器生成的。

传递 RNAs (mRNAs) 针对特定的细胞部位来控制基因表达. 本综述探讨了RNA元素,蛋白质和颗粒如何使mRNA运输成为精确的细胞调节.

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Mining Spatial Transcriptomics Datasets using DeepSpaceDB
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Mining Spatial Transcriptomics Datasets using DeepSpaceDB

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

相关实验视频

Last Updated: Jun 25, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 遗传学 是一个遗传学.

背景情况:

  • mRNA局部化是空间和时间基因表达控制的关键机制.
  • 已知很大一部分mRNA积聚在特定的细胞质区域.
  • 了解mRNA运输对于理解细胞功能和发育至关重要.

研究的目的:

  • 审查控制细胞内mRNA局部化的机制.
  • 要突出cis作用元素,RNA结合蛋白和mRNA颗粒的作用.
  • 讨论分子电机和细胞骨轨道在mRNA运输中的参与.

主要方法:

  • 关于mRNA局部化的最近研究的文献综述.
  • 分析涉及RNA运输的分子机制.
  • 合成关于RNA结合蛋白和颗粒形成的信息.

主要成果:

  • 鉴定cis作用RNA元素对于指导mRNA定位至关重要.
  • 阐明RNA结合蛋白在调解mRNA运输中的作用.
  • 描述mRNA颗粒组装及其沿细胞骨架的运动依赖的运动.

结论:

  • mRNA局部化是一个复杂的过程,对细胞功能至关重要.
  • RNA元素,蛋白质和颗粒之间的相互作用促进了向的mRNA传递.
  • 对mRNA传输机制的进一步研究可以揭示对各种生物过程和疾病的见解.