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胚胎发育期间的FGF信号调节了不同表皮的乳毛长度
Judith M Neugebauer1, Jeffrey D Amack, Annita G Peterson
1Department of Neurobiology and Anatomy, University of Utah School of Medicine, Eccles Institute of Human Genetics, Building 533, Room 3160, 15 North 2030 East, Salt Lake City, Utah 84112-5330, USA.
Nature
|February 27, 2009
概括
纤维细胞生长因子 (FGF) 信号传递对于调节脊椎动物胚胎发育中的乳毛长度和功能至关重要. 这种途径通过影响内运输基因来控制乳毛长度,从而影响各种组织.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 乳毛是重要的细胞表面器官,参与胚胎发育和细胞信号传递.
- 控制乳毛长度的细胞-细胞信号通路在很大程度上是未知的.
研究的目的:
- 研究纤维细胞生长因子 (FGF) 信号在调节乳毛长度和功能的作用.
- 阐明FGF信号控制乳毛长度的分子机制.
主要方法:
- 在斑马鱼和Xenopus胚胎中的FGF受体1 (Fgfr1) 的Morpholino敲除.
- 主导负FGF受体 (DN-Fgfr1) 的表达.
- 使用SU5402.2.使用FGF信号的药理抑制.
- 对乳毛长度,流体流动和基因表达的分析 (foxj1, rfx2, ift88).
主要成果:
- 抑制FGF信号传递 (Fgfr1 knockdown, DN-Fgfr1, SU5402) 在各种组织中 (Kupffer囊泡,眼囊泡,前管道,胃结膜板) 持续减少毛长度.
- 斑马鱼的小缩短的长度Kupffer的囊泡扰乱了方向流体流,这对于左右轴形成至关重要.
- FGF信号通过Fgf8/Fgf24-Fgfr1-内鞭状运输通路调节毛长度,降低foxj1,rfx2和ift88表达的调节.
结论:
- 在多个脊椎动物组织中,FGF信号传递在调节乳毛长度方面发挥着基本且保留的作用.
- FGF信号的失调可能会导致发育缺陷和通过功能受损的疾病.
- 这些发现揭示了一条保存的FGF介导的途径,通过内运输控制毛长度.
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