复合氨酸-蛋白质聚合物的光启动破坏
Gregory P Robbins1, Masaya Jimbo, Joe Swift
1School of Engineering and Applied Sciences, Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Journal of the American Chemical Society
|March 3, 2009
概括
蛋白质聚合物囊泡在充满费里和色氨酸二聚体时变得光响应. 暴露于光线会导致变形和破坏,使封装分子的受控释放成为可能.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 纳米技术 纳米技术
背景情况:
- 两性双块共聚物形成双层囊泡 (聚合体).
- 像费里丁这样的蛋白质可以被纳入聚合体,从而诱导不对称的结构.
- 光响应材料为材料操纵提供受控触发器.
研究的目的:
- 为了研究充满费里和二烯二聚体 (PZn(2) 的聚合体的光响应行为.
- 了解费里和PZn的协同作用对聚合体结构和功能的作用.
- 为了证明这些工程气囊的光诱导货物释放.
主要方法:
- 同焦显微镜来追踪标记的费里和PZn(2) 的定位.
- 在光漂白后的光回收 (FRAP),以评估分子扩散.
- 微管吸入实验,以测量囊泡的机械性能.
- 一个模型小分子 (biocytin) 的封装和光诱导释放.
主要成果:
- 费里丁和PZn(2) 的结合导致了不对称的聚合体结构.
- 光辐射诱导了高分子体的快速变形和破坏.
- 费里丁和PZn(2) 显示缓慢扩散,表明膜关联.
- 囊泡表现出增加的弹性模块和改变的曲刚性.
- 被封装的生物在暴露于光线时被释放出来.
结论:
- 费里和PZn(2) 之间的协同作用产生了光响应的聚合体.
- 费里丁促进变形,而PZn(2) 收集光能进行局部加热.
- 这种蛋白质聚合物膜相互作用是一种适用于其他蛋白质的一般现象.
- 光触发的变形和释放显示了可控药物递送应用的潜力.
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