对高度保存的流感病毒表位体的抗体识别
Damian C Ekiert1, Gira Bhabha, Marc-André Elsliger
1Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science (New York, N.Y.)
|March 3, 2009
概括
一种新的人类抗体CR6261针对流感血质素的保护区域,为各种流感亚型和未来的流行病提供潜在的广泛保护. 这一发现有助于开发改进的流感疫苗和治疗方法.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
背景情况:
- 流感病毒对全球公共卫生构成重大威胁.
- 目前的疫苗对不同的或新型流感菌株提供有限的保护.
- 广泛中和的针对保存表位的抗体是普遍流感保护所需的.
研究的目的:
- 确定大致中和人类抗体CR6261对流感血凝素 (HA) 的结构基础.
- 为开发通用流感疫苗和治疗方法确定一个保存的表象.
主要方法:
- 来自大流行H1N1和禽流感H5N1病毒的HA复合的CR6261 Fab的晶结构的确定.
- 高分辨率结构分析 (2.2和2.7安格斯特罗姆).
主要成果:
- CR6261识别了HA (血凝素) 的膜近端干中的一个保存的螺旋区域.
- 这种表位不同于那些被以前表征的流感抗体准的表位.
- 该抗体通过抑制HA介导的膜融合来中和流感病毒.
结论:
- 已识别的CR6261表位是通用流感疫苗设计的有希望的目标.
- 这一发现有助于开发基于抗体的流感治疗疗法.
- 类似CR6261的抗体可以提供针对各种流感亚型和流行病菌株的免疫力.
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