在DNA骨干上的多光体:一组多色的标签在一个波长激发激发
Yin Nah Teo1, James N Wilson, Eric T Kool
1Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Journal of the American Chemical Society
|March 4, 2009
概括
研究人员开发了新的多色,水溶性寡氧化化物 (ODF) 染料. 这些ODF可以同时被紫外线激发,并显示出生物标记和跟踪在生物系统中的潜力.
科学领域:
- 有机化学 有机化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 光脱氧化物单体 (化物) 可以组装成类似DNA的寡氧氧化物 (ODF).
- 这些ODF在碳化合物和异环芳香光体之间表现出独特的物理和电子相互作用.
研究的目的:
- 为了识别一种多色的水溶性ODF染料.
- 描述它们的光谱特性和同时激发的潜力.
- 评估它们在细胞和整个生物体模型中作为生物标签的实用性.
主要方法:
- 在PEG聚乙烯珠子上构建4096个四聚烯ODF图书馆.
- 使用长通波器进行选,以观察可见的颜色.
- 对23种具有不同染色体数量和间隔单体的选定ODF进行了再合成和表征.
- 辐射最大值和光谱变化的光谱分析.
- 使用人类瘤 (HeLa) 细胞和活斑马鱼胚胎进行初步体外试验.
主要成果:
- 识别ODF染料的最大发射范围从376nm到633nm,覆盖紫色到红色的表面颜色.
- 通过长波长紫外线 (340-380纳米) 同时激发所有已识别的染料.
- 斯佩克特拉揭示了堆叠的单体之间广泛的电子相互作用,受到最近邻居序列的影响.
- ODFs证明了细胞膜透和在HeLa细胞中的细胞质局部化.
- 在斑马鱼胚胎中,ODF显示组织透,生物稳定性,没有明显的毒性.
结论:
- 已经开发出了一套多功能,水溶性,多色的ODF染料.
- 这些染料适用于同时激发,并且由于单体相互作用而表现出独特的光谱特性.
- ODF染料在先进的生物标签方面表现有前途,使复杂的生物系统和动态环境中的多颜色跟踪成为可能.
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