一种基因编码的生物发光指标,用于活细胞中的通道活性
Asami Kaihara1, Akihiko Sunami, Junko Kurokawa
1Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Journal of the American Chemical Society
|March 11, 2009
概括
研究人员使用基因编码的Gaussia luciferase创建了一个用于 (Na+) 通道的新型生物发光指示器. 该工具提供了一种敏感且无毒的方法来研究Na+通道活性,并有助于用于各种疾病的药物发现.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 电压关闭的 (Na+) 通道对于细胞电信号传输至关重要.
- Na+通道的功能障碍与心律失常,和疼痛有关.
- 监测Na+通道活动的现有方法在灵敏度或吞吐量方面可能受到限制.
研究的目的:
- 开发一种新的,基因编码的生物发光指标,用于检测Na+通道活动.
- 评估该指标在药物发现中的高通量查中的有用性.
- 为了实时监测活细胞中的Na+通道功能.
主要方法:
- 基因编码一个Na+敏感的Gaussia luciferase与电压关闭的Na+通道融合.
- 使用发光测试来检测通过道的Na+流.
- 评估指示器的灵敏度,背景发光,毒性和动态范围.
主要成果:
- 成功开发了一种基因编码的生物发光Na+通道指示器.
- 展示高灵敏度和低背景发光度.
- 证实指标的无毒性和广泛的线性动态范围.
- 对于高通量选应用的潜力.
结论:
- 开发的指标为研究Na+通道功能提供了灵敏和多功能工具.
- 这项技术有望加速针对Na+通道的药物发现.
- 它为监测神经元活动和细胞电生理学 in vivo提供了一个有价值的系统.
相关概念视频
Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...


