相关实验视频
Updated: Jun 25, 2026

08:02
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
Published on: October 19, 2013
与G蛋白结合的受体激酶相互作用蛋白-1是肺血管发育所需的
Jinjiang Pang1, Ryan Hoefen, Gloria S Pryhuber
1Aab Cardiovascular Research Institute and the Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
Circulation
|March 11, 2009
概括
与G蛋白结合的受体激酶相互作用蛋白-1 (GIT1) 对肺部发育至关重要. GIT1淘汰赛小鼠显示肺血管发育受损,突出其在血管内皮生长因子 (VEGF) 信号传递中的作用.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- G蛋白结合受体激酶相互作用蛋白-1 (GIT1) 是一种多域支架蛋白,参与各种细胞过程.
- 以前的研究强调了GIT1在受体内化,焦点粘附重塑和信号通路中的作用.
- 在这项研究之前,没有实体研究研究过GIT1的功能.
研究的目的:
- 为了研究GIT1.1的基本体内功能.
- 确定GIT1在肺血管发育和血管生成中的作用.
- 阐明GIT1在血管内皮生长因子 (VEGF) 信号传递中的机制.
主要方法:
- 一个传统的GIT1淘汰赛小鼠模型的生成.
- 对GIT1淘汰赛小鼠进行病理检查,重点关注肺部发育.
- 研究从淘汰赛小鼠培养的内皮细胞和肺组织中的VEGF信号通路.
- 评估脂酶- (PLCgamma) 和细胞外信号调节激酶1/2 (ERK1/2) 的酸化和活性.
主要成果:
- 在GIT1淘汰赛小鼠中,孕期死亡率显著 (约为60%).
- 观察到的主要异常是肺部发育受损,肺血管缩小,气泡空间增加.
- 在内皮细胞中,GIT1的抑制降低了PLCgamma和ERK1/2.2.的VEGF介导酸化.
- 在产后GIT1淘汰赛小鼠的肺部观察到减少PLCgamma和ERK1/2活性.
结论:
- GIT1在肺血管发育中起着至关重要的作用.
- GIT1调节了PLCgamma和ERK1/2信号通路的VEGF诱导激活.
- 这些发现为GIT1在血管生成中建立了新的体内功能.
相关概念视频
Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
IP3/DAG Signaling Pathway
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
Pulmonary Hypertension: Classification and Pathogenesis
Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
There are various classifications for PH, each relating to different underlying causes and also...
Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
