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Updated: Jun 24, 2026

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
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Published on: January 7, 2019

可光激活绿色光蛋白的结构和机制

J Nathan Henderson1, Rinat Gepshtein, Josef R Heenan

  • 1Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA.

Journal of the American Chemical Society
|March 13, 2009
PubMed
概括

可光激活的绿色光蛋白 (PA-GFP) 晶体结构显示,UV诱导的Glu222脱碳化驱动光激活. 这种机制与稳定突变相结合,比野生型GFP增强了100倍的对比.

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability10:31

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