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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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在蛋白质 G B1 域中,盐桥与 lysines 的表征

Jennifer H Tomlinson1, Saif Ullah, Poul Erik Hansen

  • 1Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN UK.

Journal of the American Chemical Society
|March 14, 2009
PubMed
概括

核磁共振 (NMR) 研究显示,蛋白G中的表面溶酶不形成显著的盐桥,尽管有结晶结构证据. 质子交换率表明碳酸盐催化,而不是盐桥稳定.

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