在非对称的细胞分裂过程中,萨拉内分体中有方向的Delta和Notch贩运
F Coumailleau1, M Fürthauer, J A Knoblich
1Department of Biochemistry, University of Geneva, 30 Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland.
Nature
|March 20, 2009
概括
一个新的机制揭示了Notch信号是如何在细胞分裂之前被引导的. 内部化的Notch和Delta蛋白质不对称地被运输到子细胞中,从而偏向发展感官器官的信号结果.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 在不对称的细胞分裂后,痕信号对细胞命运的确定至关重要.
- 定向的Notch信号依赖于Delta和Notch效应者的差异性内细胞化.
- 之前的模型专注于子细胞 (pIIa和pIIb) 中的内细胞分裂.
研究的目的:
- 阐明一个新的定向Notch信号传输机制.
- 调查细胞分裂过程中Notch和Delta细胞内贩运的作用.
- 了解在不对称内细胞分裂之前,信号偏差是如何建立的.
主要方法:
- 在感官器官前体 (SOPs) 中追踪内化Delta和Notch蛋白.
- 在SOP转化过程中,分析蛋白质贩运到萨拉阳性内体的情况.
- 评估子细胞 (pIIa和pIIb) 中的Notch切割和激活.
主要成果:
- 在分割SOP中,将Delta和Notch流量内部化到Sara内体.
- 含有Notch和Delta的Sara内分体移动到中央,然后到pIIa子细胞.
- 隙裂变发生在Sara内基因组中的pIIa中,依赖于玛分泌酶和Delta内部化.
结论:
- 一个新的机制通过内部化带和受体的不对称贩运来指导Notch信号.
- 萨拉内分体的这种非对称的前转移性准偏向了Notch向pIIa细胞发出信号.
- 这种机制在子细胞的差异性内细胞分裂之前建立了信号不对称性,影响了的感觉器官发育.
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