相关实验视频
Updated: Jun 24, 2026

10:59
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
极端的基因组修复
Rodrigo S Galhardo1, Susan M Rosenberg
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Cell
|March 24, 2009
概括
细菌Deinococcus radiodurans可以在极端辐射损伤后修复其破碎的基因组. 这项研究揭示了令人惊的普通修复步骤,质疑为什么其他生物体不能进行如此极端的基因组修复.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 辐射生物学 辐射生物学
背景情况:
- 迪诺科克 (Deinococcus radiodurans) 对电离辐射具有显著的抗性.
- 高剂量的辐射将细菌的基因组碎成许多碎片.
相关概念视频
Nucleotide Excision Repair
Overview
Nucleotide Excision Repair
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair
Overview
Base Excision Repair
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
Base Excision Repair
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
Long-patch Base Excision Repair
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
