在5.5A分辨率下,人类结合体U1 snRNP的晶体结构
Daniel A Pomeranz Krummel1, Chris Oubridge, Adelaine K W Leung
1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.
Nature
|March 28, 2009
概括
确定了人类结合体U1小核核糖核蛋白颗粒 (snRNP) 的结构,揭示了复杂的子单元相互作用. 这为U1 snRNP组件和5'拼接部位识别机制提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 结合体U1小核核核糖核蛋白颗粒 (snRNP) 对于mRNA前拼接至关重要.
- U1 snRNP 识别了 5' 结合部位,启动了结合体组装以进行内子去除.
研究的目的:
- 为了确定人类U1 snRNP.功能核心的高分辨率结构.
- 阐明U1 snRNP子单位之间的复杂相互作用.
- 为了了解U1 snRNP组件和5'拼接部位识别.
主要方法:
- 用5.5A分辨率的冷电子显微镜生成电子密度图.
- 在地图中构建RNA结构.
- 单个蛋白质的特定标签,以在地图上定位它们.
- 整合数据以建模七种Sm蛋白,U1-C和U1-70K.
主要成果:
- U1 snRNP 功能核心的详细结构.
- 在U1 snRNP子单位之间交互的等级网络.
- U1-70K的N端多体跨度为180A,与Sm蛋白核心和U1-C相互作用.
结论:
- 确定的结构显示了U1 snRNP.的复杂组织.
- 这些发现为U1 snRNP的组装途径提供了洞察力.
- 由U1 snRNP提出了一种潜在的5'拼接部位识别机制.
相关概念视频
RNA Structure
81.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
81.3K
RNA Splicing
61.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
61.5K
RNA Structure
29.8K
No description available
29.8K
RNA Splicing
20.3K
No description available
20.3K
RNA Structure
8.3K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.3K
Chromatin Structure and RNA Splicing
3.7K
No description available
3.7K


