相关实验视频
Updated: Jun 24, 2026

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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
核激素受体调节的microRNAs控制发育进展
Axel Bethke1, Nicole Fielenbach, Zhu Wang
1Huffington Center on Aging, Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
概括
核受体和microRNAs在发育过程中控制基因表达. 这项研究揭示了核受体DAF-12如何使用微RNA作为分子开关,以在激素反应中在发育阶段之间过渡.
科学领域:
- 发展生物学 发展生物学
- 分子内分泌学分子内分泌学
- 基因规则 基因规则
背景情况:
- 核受体 (NRs) 调节基因表达,以应对视网和类固醇等小分子,控制组织发育.
- 微RNA (miRNA) 是小型非编码RNA,可在转录后调节基因表达,在发育和疾病中至关重要.
研究的目的:
- 研究Caenorhabditis elegans核受体DAF-12在调节microRNA表达中的作用.
- 阐明DAF-12及其连接物通过微RNA控制发育过渡的机制.
主要方法:
- 对Caenorhabditis elegans核受体DAF-12及其类固醇配体的分析.
- 研究了let-7微RNA家族促进体的激活.
- 研究了微RNA目标基因hbl-1的下调及其对细胞分裂模式的影响.
主要成果:
- 核受体DAF-12,在与其类固醇连接体结合后,直接激活let-7微RNA家族成员的促进体.
- 这种激活导致微RNA点hbl-1的下调,该点hbl-1参与表皮干细胞进展.
- 在没有配体的情况下,DAF-12抑制了microRNA的表达,与发育停止相关.
结论:
- 微RNAs作为激素合分子开关的组件.
- 这种开关机制使得早期的开发程序可以终止,以便启动后来的开发程序.
- 发现了一种新的调节途径,将核激素信号与微RNA介导的发育时间联系起来.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Transducer Mechanism: Nuclear Receptors
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

