扩展遗传字母的转录
Young Jun Seo1, Shigeo Matsuda, Floyd E Romesberg
1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Journal of the American Chemical Society
|April 9, 2009
概括
研究人员使用非自然的基对创建了一个半合成有机体,具有扩展的遗传密码. 这些合成DNA基对,如d5SICS:dNaM,被有效地复制和转录,为新的生物技术应用铺平了道路.
科学领域:
- 合成生物学 合成生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 用人工基因对扩展遗传字母为生物技术提供了潜力.
- 不自然的基对 (UBP) 复制的效率和保真度越来越高.
- 核基形状和疏水性可以决定DNA复制.
研究的目的:
- 在两个链上下文中研究UBP的转录.
- 为了确定水相互作用是否足以进行转录.
- 评估UBP对体外扩展遗传字母的适用性.
主要方法:
- 使用T7RNA聚合酶进行转录测试.
- 使用的非自然核酸是d5SICS,dMMO2和dNaM.
- 评估了UBP的复制和转录效率和真实性.
主要成果:
- 这两种d5SICS:dMMO2和d5SICS:dNaM都被T7RNA聚合酶选择性转录.
- 在这两种情况下,d5SICS:dNaM的转录效率与自然基对相比仅略有降低.
- 转录不需要结合;疏水力足够.
结论:
- 这些UBPd5SICS:dMMO2和d5SICS:dNaM显示出高效和选择性的转录.
- 这些UBP的复制和转录具有足够的准确性,可用于扩展的遗传字母.
- 疏水性相互作用可以取代复制和转录的基配对中的键.
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