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Updated: Jun 24, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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多复合三明治免疫试验使用电化学发光成像,分辨率在单珠水平

Frédérique Deiss1, Christopher N LaFratta, Matthew Symer

  • 1Universite Bordeaux, Institut des Sciences Moleculaires, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France.

Journal of the American Chemical Society
|April 14, 2009
PubMed
概括

一个新的基于珠子的微阵列平台,可以使用电生成化学发光 (ECL) 同时检测多个抗原. 这项技术允许使用单个珠子分辨率进行高度多重测定,展示了其在诊断方面的潜力.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.