在细胞染色体c中直接观察配体动态
Megan C Thielges1, Jörg Zimmermann, Floyd E Romesberg
1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Journal of the American Chemical Society
|April 14, 2009
概括
这项研究揭示了快速的CO联结体动态,而不是蛋白质折叠,驱动CO光解离后马心细胞染色体c的早期光谱变化. 这些发现影响了对蛋白质折叠动力学和链间扩散速率的解释.
科学领域:
- 生物化学 生物化学
- 蛋白质动力学 蛋白质动力学
- 频谱学是一种光谱学.
背景情况:
- 马心细胞染色体c (cyt c) 是蛋白质折叠研究的模型,因为它具有血红组.
- 之前的研究解决了CO光解离后的四个过渡,归因于蛋白质折叠和血结合.
研究的目的:
- 为了明确确定涉及一氧化碳 (CO) 连接体动态的光解离后步骤.
- 为了澄清早期的光谱变化是否反映了蛋白质折叠或CO联结体行为.
主要方法:
- 使用步骤扫描里埃变换红外光谱 (FT-IR) 来监测CO振动.
- 研究了从CO结合的未折叠细胞c.c.中进行的CO光解离.
主要成果:
- 50-100微秒的时间尺度过程与CO联体动力学有关,而不是蛋白质动力学.
- 观察到的光谱变化涉及CO重新结合或结合的CO合体环境的改变.
- 在快速结合CO重组后的His18重组是观察到变化的可能解释.
结论:
- 50-100微秒的时间常数不应用于测量链间扩散速率.
- 强调在蛋白质折叠研究中需要具有高结构分辨率的探针.
- 在细胞染色体c中区分蛋白质折叠事件和血红结动态.
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