使用工程微生物从生物质中合成甲基化物
Travis S Bayer1, Daniel M Widmaier, Karsten Temme
1Department of Pharmaceutical Chemistry, University of California, San Francisco, MC 2540, Room 408C, 1700 4th Street, San Francisco, California 94158-2330, USA.
Journal of the American Chemical Society
|April 22, 2009
概括
研究人员为工业生产设计了甲基化物转移酶 (MHT) 酶. 这一进步使农业废物可可持续合成甲基化物,为化学和燃料前体提供更绿色的替代品.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 生物催化剂是一种生物催化剂.
背景情况:
- 甲基化物是重要的工业化学品和燃料前体.
- 生物体的自然生产产量对于工业需求来说太低了.
- 甲基化物转移酶 (MHT) 酶催化甲基组从S-adenosyl metionin (SAM) 转移到化物.
研究的目的:
- 识别和设计新的甲基化物转移酶 (MHT) 酶,以实现高效的工业生产.
- 利用非食品农业生物质开发一种可持续的甲基化物生物生产平台.
主要方法:
- 合成元基因组方法合成89个假定的MHT基因.
- 在大肠杆菌中对MHT库进行选,以确定化物生产率.
- 在Saccharomyces cerevisiae中高活性MHT的工程和与Actinotalea fermentans的共同培养.
主要成果:
- 合成的MHT基因中有56%对化物,85%对化物,69%对化物表现出活性.
- 在酵母中改造的MHT从葡萄糖和砂糖中获得了190 mg/L-h的生产力.
- 通过共同培养,成功地从未加工的纤维素生物质 (交换草,玉米,包,) 中生产甲基化物.
结论:
- 这项研究表明了工程MHT酶在工业甲基化物生产中的潜力.
- 一个共生共养系统可以利用各种农业废物流.
- 这种方法提供了一种可持续和可扩展的方法,用于从非食品生物质中生产有价值的化学物质.
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