相关实验视频
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
Published on: June 16, 2022
微RNA在发育过程中赋予了对环境波动的强度
Xin Li1, Justin J Cassidy, Catherine A Reinke
1Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, Illinois 60208, USA.
Cell
|April 22, 2009
概括
微RNAmiR-7有助于稳定发育网络以应对环境变化. 这种保存的微RNA (miRNA) 缓冲基因调节,确保跨物种的发育稳定性.
科学领域:
- 进化发育生物学 进化发育生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 微RNAs (miRNAs) 是基因表达的关键调节者,在各种物种中得到保护.
- 微RNAmiR-7在无脊椎动物到人类之间高度保存.
- 然而,由miR-7调节的特定基因在物种之间可能会有所不同,这表明血统特定的角色.
研究的目的:
- 为了研究微RNA-目标关系的进化意义.
- 探索血统受限目标在微RNA功能中的作用.
- 了解miR-7如何为发展性监管网络的稳定性做出贡献.
主要方法:
- 研究了Drosophila的两个特征很好的发育调节网络.
- 在反和前循环中分析了miR-7的功能.
- 将监管网络置于温度波动之下,以评估稳定性.
主要成果:
- miR-7参与多个相互锁定的反和前循环.
- miR-7对于在环境压力 (温度波动) 下保持监管稳定至关重要.
- 证明miR-7作为缓冲对发育网络中扰动的缓冲.
结论:
- 像miR-7这样的保存的微RNA可以形成新的遗传相互作用,以缓冲发育程序.
- 这些相互作用增强了对环境变化的强度.
- miR-7在确保发育稳定性和适应性方面发挥着至关重要的作用.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability