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Updated: Jun 23, 2026

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在单个分子水平上检测DNA孔转移的"信号启动"检测

Tadao Takada1, Yuichiro Takeda, Mamoru Fujitsuka

  • 1The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan.

Journal of the American Chemical Society
|April 25, 2009
PubMed
概括

研究人员开发了一种用于检测单个DNA分子中的DNA孔转移 (DNA HT) 的新方法. 这种技术使得使用光光谱学能够灵敏而准确地识别DNA信息和基对不匹配.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...