发现了昆虫和人类登革热病毒宿主因素
October M Sessions1, Nicholas J Barrows, Jayme A Souza-Neto
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Nature
|April 28, 2009
概括
研究人员使用全基因组RNA干扰屏幕确定了116种昆虫宿主因子,这些因素对登革热病毒 (DENV) 传播至关重要. 其中许多因素,包括42个人类同类,都与DENV感染有关,提供了新的控制策略.
科学领域:
- 病毒学 病毒学
- 遗传学 是一个遗传学.
- 昆虫学 昆虫学是一门学科.
背景情况:
- 登革热是一种重要的树冠病毒疾病,由于高发病率和缺乏特定治疗方法,它构成了全球健康威胁.
- 识别宿主因素对于理解和控制登革热病毒 (DENV) 复制至关重要.
- 之前已经确定了DENV的有限的人类和昆虫宿主因素.
研究的目的:
- 为了确定新的昆虫宿主因子,对于DENV-2传播至关重要.
- 研究昆虫和人类宿主之间的这些宿主因素的保护.
主要方法:
- 在Drosophila melanogaster细胞中进行全基因组RNA干扰 (RNAi) 查,使用22,632个双链RNAs的库.
- 确定候选登革热病毒宿主因子 (DVHFs).
- 在人体细胞中准短干扰RNA (siRNA) 选以验证保存的DVHFs.
主要成果:
- 查发现了Drosophila中的116个候选DVHF,其中大多数以前与DENV传播无关.
- 其中82种二体DVHF具有可识别的人类同类.
- 42个人类同类被证实是人类的DVHF,表明了显著的保护.
结论:
- 这项研究揭示了DENV传播所需的大量以前未知的昆虫和人类宿主因素.
- 已识别的保存宿主因素为控制昆虫载体和哺乳动物宿主中DENV提供了潜在的目标.
- 这些发现为针对登革热的新战略铺平了道路.
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