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相关概念视频

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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相关实验视频

Updated: May 7, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Bmi1调节线粒体功能和DNA损伤反应途径.

Jie Liu1, Liu Cao1, Jichun Chen2

  • 1Translational Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature
|May 1, 2009
PubMed
概括

缺乏Bmi1的小鼠显示线粒体功能受损,氧化应激增加,导致DNA损伤. 抗氧化剂治疗或阻断DNA修复途径改善了这些Bmi1缺乏的小鼠.

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相关实验视频

Last Updated: May 7, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

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Published on: September 28, 2012

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

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科学领域:

  • 干细胞生物学 干细胞生物学
  • 分子生物学分子生物学
  • 线粒体功能 线粒体功能

背景情况:

  • 多胞体抑制剂Bmi1对于干细胞的自我更新和发育至关重要.
  • Bmi1 缺乏导致 Ink4a/Arf 位点去抑制,导致发育异常.
  • 在这些过程中Bmi1的作用背后的确切机制尚未完全理解.

研究的目的:

  • 研究Bmi1在细胞代谢和DNA损伤中的作用.
  • 探索Bmi1缺乏对线粒体功能和活性氧物种 (ROS) 的影响.
  • 为了确定是否准线粒体功能障碍或DNA损伤途径可以拯救Bmi1缺乏的表型.

主要方法:

  • 从Bmi1-缺乏 (Bmi1(-/-)) 的小鼠中获得的细胞的分析.
  • 评估线粒体功能和细胞内ROS水平.
  • 用N-乙半氨酸 (抗氧化剂) 进行药理治疗和基因删除Chk2 (DNA损伤反应途径).

主要成果:

  • Bmi1(-/-) 细胞表现出线粒体功能受损和ROS水平升高.
  • 增加的ROS触发了Bmi1缺乏细胞中的DNA损伤反应途径.
  • 与N-乙半氨酸治疗或Chk2删除改善了许多与Bmi1 ((-/-) 相关的缺陷.

结论:

  • Bmi1在维护线粒体功能和氧化还原平衡中起着意想不到的作用.
  • 细胞代谢是由Polycomb蛋白与干细胞和原生细胞功能一起协调调节的.
  • 准线粒体功能障碍和DNA损伤途径为Bmi1相关疾病提供了潜在的治疗策略.