哺乳动物表达的红外光蛋白是从细菌植物染色体中改造出来的
Xiaokun Shu1, Antoine Royant, Michael Z Lin
1Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0647, USA.
Science (New York, N.Y.)
|May 9, 2009
概括
研究人员开发了用于动物全身成像的新型红外光蛋白 (IFP). 这些新型蛋白质克服了传统光蛋白的局限性,使组织深处能够更好地可视化.
科学领域:
- 分子生物学分子生物学
- 生物物理学的生物物理.
- 生物技术是生物技术.
背景情况:
- 光蛋白 (FPs) 是分子和细胞生物学中的重要工具.
- 传统的FP在体内成像中具有有限的组织透能力.
- 激发光透是全身动物研究的一个关键挑战.
研究的目的:
- 为改进体内成像设计新型红外光蛋白 (IFP).
- 为了克服可见光蛋白在深层组织透中的局限性.
- 开发一种适用于全身动物成像的光蛋白系统.
主要方法:
- 设计了一个来自Deinococcus radiodurans的细菌菌.
- 作为染色体,纳入了 biliverdin.
- 开发出了单体红外光蛋白 (IFP).
主要成果:
- 在684/708 nm. 达到激发/排放最大值的IFP.
- 获得了高的灭绝系数 (>90,000 M(-1) cm(-1)) 和量子产量 (0.07).
- 在哺乳动物细胞和小鼠中成功表达和结合 biliverdin.
结论:
- 工程IFP表现出有利的光谱特性和体内表达.
- IFPs为全身成像应用提供卓越的组织透能力.
- 开发的IFP作为未来蛋白质工程的多功能平台.
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