相关实验视频
Updated: Jun 19, 2026
07:59
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
一个纳米级DNA盒的自组装,具有可控制的盖子
Ebbe S Andersen1, Mingdong Dong, Morten M Nielsen
1Danish National Research Foundation: Centre for DNA Nanotechnology.
Nature
|May 9, 2009
概括
研究人员创建了一个3DDNA原木盒,用DNA钥匙打开. 这种纳米容器为逻辑传感器和纳米货物释放等应用提供了受控访问.
科学领域:
- 纳米技术 纳米技术
- 生物技术是生物技术.
- 结构生物学 结构生物学
背景情况:
- DNA的自组装特性使得纳米结构的自下而上的构建成为可能.
- 基因原始设计使得2D可定位结构能够实现精确的纳米材料排列.
- 一个关键的目标是开发完全可定位的3D DNA纳米结构.
研究的目的:
- 为了将DNA原木方法扩展到三维.
- 为了创建一个功能,可定位的3D DNA纳米结构.
- 为了证明DNA纳米容器的受控打开.
主要方法:
- 利用DNA原木技术构建了一个3DDNA盒.
- 使用低温传输电子显微镜 (cryo-TEM),小角度X射线散射 (SAXS) 和原子力显微镜 (AFM) 进行了纳米结构的特征.
- 使用光共振能量转移 (FRET) 来监控打开机制.
主要成果:
- 成功构建了一个可定位的3DDNA盒 (42 x 36 x 36 nm3).
- 证明使用特定的DNA"钥匙"可以打开DNA盒.
- 检查结构完整性,并通过光学监控盖子打开过程.
结论:
- 基因原形法可以扩展到创建复杂的3D纳米结构.
- 可定位的DNA盒提供了对其内部的受控访问.
- 潜在的应用包括多序逻辑传感器和控制纳米货物释放系统.
相关概念视频
00:58Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
00:58DNA Packaging
Overview
00:58DNA Packaging
Overview
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...